RNA Preparation and Purification
New England Biolabs, Inc. Monarch® Spin RNA Cleanup Kit (500 ug) - 10 preps
The Monarch Spin RNA Cleanup Kit (500 ug) reliably purifies up to 500 ug of concentrated, high-quality RNA (>25 nt) from enzymatic reactions and in vitro transcription (IVT) reactions. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our columns help ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 50 ul. Unwanted NTPs and short RNA fragments are removed, ensuring highly pure RNA transcripts following IVT/RNA synthesis. Eluted RNA is ready for use in a variety of downstream applications including RT-PCR, RNA Library Prep for NGS and RNA labelling. The protocol can also be modified to enable the purification of smaller RNA fragments (>= 15 nt). Monarch Spin RNA Cleanup Kits are also available for 10 ug and 50 ug binding capacities. Columns and buffers are also available separately for convenience
Zymo Research Corporation Quick-RNA 96™ Kit (2 x 96 Preps)
The Quick-RNA 96 Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes. The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
Macherey-Nagel NucleoSpin RNA Clean-up XS, 10 preps
NucleoSpin RNA Clean-up XS (10) 10 preps for the clean-up of total RNA - NucleoSpin RNA XS Columns, Collection Tubes, buffers
Roche Diagnostics KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin, 96 Libraries, RNA-Seq, 25 ng - 1 μg input RNA, for high- and low-quality RNA, including FFPE tissue
The KAPA RNA HyperPrep Kit with RiboErase (HMR) Globin is a streamlined, strand-specific solution designed for high-quality RNA-seq library preparation from various RNA inputs. This kit reduces preparation time and combines enzymatic steps while ensuring comprehensive transcriptome coverage by efficiently depleting ribosomal RNA and globin mRNA from samples. It is suitable for both manual and automated workflows on Illumina platforms and is compatible with degraded samples, making it an ideal choice for various research applications.
- Streamlined library preparation reduces time
- Strand-specific RNA-seq libraries
- Compatible with low-input and degraded samples
- Supports both manual and automated workflows
- Efficient rRNA and globin mRNA depletion
- High throughput and consistent results
- Designed for Illumina platforms
- Kit accommodates 96 libraries
- Research use only
Zyagen Labs Human Universal Reference Total RNA extracted from wide variety of male and female HUMAN tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR & many other applications. 0.05mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
Zyagen Labs Mouse C57 Brain Total RNA, 0.05 mg
Mouse C57 Whole Brain Total RNA is extracted from freshly harvested normal healthy tissue using classical guanidine isothiocyanate–phenol: chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase-1 to remove residual DNA, precisely quantified by NanoDrop, and stored at -80oC.
Zyagen Labs Human liver total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN LIVER tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Human testis total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN TESTIS tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Human lung total RNA is a high pure intact total RNA isolated from freshly harvested normal healthy HUMAN LUNG tissues, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested normal healthy tissues of single donor using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Arabidopsis total RNA is a high pure intact total RNA isolated from freshly harvested ARABIDOPSIS leaves, treated with DNase and dissolved in water. RNA is ready for immediate use in any downstream application.
Zyagen Total RNA is routinely extracted from freshly harvested plant leaves using classical guanidine isothiocyanate–phenol:chloroform extraction method which allows the rapid isolation of total RNA including microRNAs. RNA is treated with RNase-free DNase to remove residual DNA, precisely quantified, and stored at -80oC. The integrity of each RNA sample as indicated by intact ribosomal RNA is verified by denatured agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. RNA is ideal for Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, RNA differential display, microRNA studies, and purification of mRNA for library construction. Total RNA sample is provided in RNase-free water at a concentration of 1 mg/ml and shipped on dry ice.
Zyagen Labs Dog Universal Reference Total RNA extracted from wide variety of male and female Beagle DOG tissues. Made at industrial scales to minimize lot-to-lot variation. Ideal for microarray, NB, PCR and many other applications. 0.1mg/pK at 1mg/ml
Zyagen Universal Reference Total RNA is composed of highly pure intact total RNA isolated from wide variety of male & female tissues. Total RNA is extracted using modified guanidine thiocyanate method, treated with DNase & packed at a concentration of 1mg/ml. Features: High quality intact RNA without genomic DNA contamination. Consistent control for standard data set comparisons. Extracted from large variety of male & female tissues for broad gene coverage. Made at industrial scales to minimize lot-to-lot variation Quality Control: The integrity of RNA sample as indicated by intact ribosomal RNA is verified by agarose gel electrophoresis. The purity of RNA is assessed by NanoDrop (A260/A280: 1.9-2.1). Residual DNA contamination is tested by PCR. Applications: Reference RNA is ideal for microarray analysis, Northern blotting, ribonuclease protection assay, SI nuclease assay, RT-PCR/Q-PCR/RACE analysis, cDNA synthesis, microRNA studies, & purification of mRNA for library construction.
Omega Biotek Inc Mag-Bind Total RNA 96 Kit | 4 x 96 preps
The Mag-Bind Total RNA 96 Kit provides a novel technology for total RNA isolation. This kit allows the rapid and reliable isolation of high-quality total cellular RNA and viral RNA from a wide variety of cells and tissues. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution resulting in increased binding kinetics and binding efficiency. Particles are also completely re-suspended during the wash steps of the purification protocol, which improves the removal of contaminants and increases nucleic acid purity. Mag-Bind Total RNA 96 Kit procedure can be fully automated with most robotic workstations.